Bcl-X Antibody Cocktail orb385712 from Biorbyt

Description

Recognizes a protein of 27kDa, identified as the Bcl-X protein. This mAb cocktail shows no cross-reaction with Bcl-2 or Bax protein. Bcl-X has two isoforms, Bcl-XL (long), a 241 amino acid protein which suppresses cell death. And Bcl-XS (short), a 178 amino acid protein lacking a 63 amino acid domain which functions as a dominant inhibitor of Bcl-2. This mAb reacts with both Bcl-XS and Bcl-XL proteins

Product Specs
ItemBcl-X Antibody Cocktail
CompanyBiorbyt
Price Pricing Info Supplier Page
Catalog Numberorb385712
Quantity20 µg, 100 µg
ApplicationsIHC-P, WB
Conjugate/TagUnconjugated
PurityProtein G Affinity Chromatography
ReactivityHuman, Mouse, Rat
TargetBcl-X
IsotypeMouse IgG2a, kappa
CloneBX006 + 2H12
HostMouse
ImmunogenAmino acids 3-14 (QSNRELVVDFLS) from the human protein were used as the immunogen for this Bcl-X antibody
NCBI Full Gene NameBCL2 like 1
NCBI Gene AliasesBCL-XL/S, BCL2L, BCLX, Bcl-X, PPP1R52
ClonalityMonoclonal
Formula0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
StorageStore the Bcl-X antibody cocktail at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide)

Specifications/Features

Application Notes: The optimal dilution of the Bcl-X antibody for each application should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Application Dilutions: Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT

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